Batch Cultivation Model for Biopolymer Production

Production of recombinant human annexin V by fed-batch cultivation

BACKGROUND Annexin V, a 35.8 kDa intracellular protein, is a Ca⁺²-dependent phospholipid binding protein with high affinity to phosphatidylserine (PS), which is a well-known hallmark of apoptosis. Annexin V is a sensitive probe for PS exposure upon the cell membrane, and used for detection of apoptotic cells both in vivo and in vitro. Large-scale production of recombinant human annexin V is wor...

Multiple model approach to modelling of Escherichia coli fed-batch cultivation extracellular production of bacterial phytase

Production of recombinant 503 antigen of Leishmania infantum chagasi using cultivation in batch and fed-batch

Background Visceral leishmaniasis is an infecto-parasitarian disease caused by obligatory intracellular protozoa belonging to the genus Leishmania, and might be lethal since there is no precocious diagnosis and proper treatment. Despite the considerable effort, there is no effective and safe vaccine for human use [1]. Genetically modified microorganisms are used industrially in general for prod...

Optimization of Single Cell Protein Production from Cheese Whey Under Batch and Continuous Cultivation

In this research the microorganism was initially isolated and selected after evaluation based on COD reduction of cheese whey and biomass production. The selected microorganism was identified as Trichosporon sp. The cultivation conditions of the microorganism were optimized under batch: temperature 30˚C initial pH = 6 aeration speed = 2 ν.ν.m and agitation rate: 800 rpm. Under these conditions,...

A new kinetic model for biogas production from co-digestion by batch mode

Coming out from the growth kinetics, the Gompertz model has been developed and considered as the best one for simulating the biogas production from anaerobic digestion. However, the model has failed to describe the starting point of the process, and no-sense of lag phase constant has been pointed out. Thus, the goal of this study is to develop a new kinetic model of biogas production with meani...